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Whole-cell MALDI-TOF Mass Spectrometry is an Accurate and Rapid Method to Analyze Different Modes of Macrophage Activation

DOI :

10.3791/50926-v

December 26th, 2013

December 26th, 2013

8,619 Views

1Unité de Recherche sur les Maladies Infectieuses Tropicales et Emergentes (URMITE), CNRS UMR 7278, INSERM U1095, Aix Marseille Université, 2Service de médecine interne, post-urgence et thérapeutique, Hôpital de la Timone

This protocol describes the use of whole-cell MALDI-TOF mass spectrometry on eukaryotic cells. Here, we illustrate the accuracy of this technique by analyzing the multiple activation states of macrophages in response to their microenvironment.

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