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Identifying Protein-protein Interaction in Drosophila Adult Heads by Tandem Affinity Purification (TAP)

DOI :

10.3791/50968-v

December 5th, 2013

December 5th, 2013

13,747 Views

1Neuroscience Center of Excellence, Louisiana State University Health Sciences Center

Drosophila is famous for its powerful genetic manipulation, but not for its suitability of in-depth biochemical analysis. Here we present a TAP-based procedure to identify interacting partners of any protein of interest from the fly brain. This procedure can potentially lead to new avenues of research.

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Protein protein Interaction

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