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JoVE Journal
Biology
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Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling
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11:55 min
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May 29th, 2011
DOI :
May 29th, 2011
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Chapters in this video
0:05
Title
1:32
Protein Expression Cysteine Mutation and mRNA Synthesis
2:07
Oocyte Removal
3:36
Oocyte Defoculation and mRNA Injection
5:05
Site-specific Fluorescence Labeling
6:07
Electrophysiology
7:26
Anisotropy Measurements and Determination of Distance Constraints
9:17
Relative Movement of Protein Subunits
10:07
Results: Current and Fluorescence Changes with Site Specific Fluorophore Labelings
11:38
Conclusion
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