Testing the Acquisition System with the Model Cell Supplied with the Amplifier
4:09
Set Up the Recording Stage and the Reference Electrode
4:48
Set Up the VDIFF Electrodes
5:51
Set Up the Current, Voltage Electrodes, Data Acquisition
6:57
Results: Representative Recording Traces
8:05
Conclusion
Transcript
Heterologous expression of connexins and innexins in Xenopus oocytes is a powerful approach for analyzing biophysical properties of gap junctions. The main advantage of our technique is to use a high-side current measuring approach that is appropr
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Here we present a protocol to express gap junction proteins in Xenopus oocytes and record junctional current between two apposed oocytes using a commercial amplifier designed for dual oocyte voltage-clamp recordings in a high side current measuring mode.