May 19th, 2014
•Here, we describe a method to analyze changes in the initiation of mRNA translation of eukaryotic cells in response to stress conditions. This method is based on the velocity separation on sucrose gradients of translating ribosomes from non-translating ribosomes.
Tags
Related Videos
Monitoring Plant Hormones During Stress Responses
Antibody Profiling by Luciferase Immunoprecipitation Systems (LIPS)
Intravital Microscopy of the Microcirculation in the Mouse Cremaster Muscle for the Analysis of Peripheral Stem Cell Migration
Analysis of Oxidative Stress in Zebrafish Embryos
Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale
Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling
Fecal Glucocorticoid Analysis: Non-invasive Adrenal Monitoring in Equids
An Easy Method for Plant Polysome Profiling
Analysis of the Gap Junction-dependent Transfer of miRNA with 3D-FRAP Microscopy
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved