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A Flow Adhesion Assay to Study Leucocyte Recruitment to Human Hepatic Sinusoidal Endothelium Under Conditions of Shear Stress

DOI :

10.3791/51330-v

11:38 min

March 21st, 2014

March 21st, 2014

14,895 Views

1NIHR Biomedical Research Unit, Centre for Liver Research, School of Immunity and Infection, University of Birmingham

Leucocyte recruitment to the liver occurs within the specialized channels of the hepatic sinusoids which are lined by unique hepatic sinusoidal endothelial cells. Phase contrast microscopy of leucocyte recruitment across human hepatic sinusoidal endothelium under conditions of physiological shear stress can facilitate the elucidation of the molecular mechanisms which underlie this process.

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Flow Adhesion Assay

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