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Assay of Adhesion Under Shear Stress for the Study of T Lymphocyte-Adhesion Molecule Interactions

DOI :

10.3791/54203-v

June 29th, 2016

June 29th, 2016

8,194 Views

1Department of Medicine, New York University School of Medicine, 2Department of Pathology, New York University School of Medicine

This flow adhesion assay provides a simple, high impact model of T cell-epithelial cell interactions. A syringe pump is used to generate shear stress, and confocal microscopy captures images for quantification. The goal of these studies is to effectively quantify T cell adhesion using flow conditions.

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