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Sample Preparation for Single Virion Atomic Force Microscopy and Super-resolution Fluorescence Imaging

DOI :

10.3791/51366-v

5:31 min

January 2nd, 2014

January 2nd, 2014

9,209 Views

1Department of Physics & Astronomy, University of Utah, 2Center for Cell and Genome, University of Utah

The attachment of virions to a surface is a requirement for single virion imaging by Super-resolution fluorescence imaging or atomic force microscopy (AFM). Here we demonstrate a sample preparation method for controlled adhesion of virions to glass surfaces suitable for use in AFM and super-resolution fluorescence imaging.

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Sample Preparation

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