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A Simple and Rapid Protocol for Measuring Neutral Lipids in Algal Cells Using Fluorescence

DOI :

10.3791/51441-v

May 30th, 2014

May 30th, 2014

34,828 Views

1Department of Chemical and Materials Engineering, University of Alberta, 2Department of Chemical and Petroleum Engineering, University of Calgary

A simple protocol to determine the neutral lipid content of algal cells using a Nile Red staining procedure is described. This time-saving technique offers an alternative to traditional gravimetric-based lipid quantification protocols. It has been designed for the specific application of monitoring bioprocess performance.

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