JoVE Logo
Faculty Resource Center

Sign In

Isolation of Human Monocytes by Double Gradient Centrifugation and Their Differentiation to Macrophages in Teflon-coated Cell Culture Bags

DOI :

10.3791/51554-v

9:32 min

September 9th, 2014

September 9th, 2014

68,403 Views

1Department of Hematology and Oncology, University Medical Center Göttingen, 2Microbial Interface Biology Group, Research Center Borstel

We present a simple and efficient protocol for the generation of human macrophages. Buffy coats are processed by double density gradient centrifugation and isolated monocytes are then differentiated to macrophages in Teflon-coated cell culture bags. This maximizes macrophage yields and facilitates cell harvesting for subsequent experiments.

Tags

Isolation

-- Views

Related Videos

article

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes

article

Highly Efficient Transfection of Human THP-1 Macrophages by Nucleofection

article

Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents

article

In Vitro Disassembly of Influenza A Virus Capsids by Gradient Centrifugation

article

Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages

article

Legionella pneumophila Outer Membrane Vesicles: Isolation and Analysis of Their Pro-inflammatory Potential on Macrophages

article

Live Imaging of Antifungal Activity by Human Primary Neutrophils and Monocytes in Response to A. fumigatus

article

Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages

article

Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages

article

Induced Differentiation of M Cell-like Cells in Human Stem Cell-derived Ileal Enteroid Monolayers

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved