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Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages

DOI :

10.3791/54244-v

June 30th, 2016

June 30th, 2016

27,206 Views

1Experimental Atherosclerosis Section, National Heart, Lung, and Blood Institute, National Institutes of Health

A protocol is presented for cell culture of macrophage colony-stimulating factor (M-CSF) differentiated human monocyte-derived macrophages. The protocol utilizes cryopreservation of monocytes coupled with their bulk differentiation into macrophages. Then harvested macrophages can then be seeded into culture wells at required cell densities for carrying out experiments.

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