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EPR Monitored Redox Titration of the Cofactors of Saccharomyces cerevisiae Nar1

DOI :

10.3791/51611-v

November 26th, 2014

November 26th, 2014

13,320 Views

1Department of Biotechnology, Delft University of Technology

The goal of this protocol is to use electron paramagnetic resonance (EPR) monitored redox titrations to identify different cofactors of Saccharomyces cerevisiae Nar1. Redox titrations offer a very robust way to obtain midpoint potentials of different redox active cofactors in enzymes and proteins.

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Keywords EPR

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