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Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein Expressed in Saccharomyces cerevisiae

DOI :

10.3791/51447-v

15:12 min

May 10th, 2014

May 10th, 2014

14,109 Views

1Faculty of Life Sciences, University of Manchester

Heterologous expression and purification of the cystic fibrosis transmembrane conductance regulator (CFTR) are significant challenges and limiting factors in the development of drug therapies for cystic fibrosis. This protocol describes two methods for the isolation of milligram quantities of CFTR suitable for functional and structural studies. 

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Cystic Fibrosis Transmembrane Conductance Regulator

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