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Expression and Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein in Saccharomyces cerevisiae

DOI :

10.3791/3860-v

14:56 min

March 10th, 2012

March 10th, 2012

18,074 Views

1Faculty of Life Sciences, University of Manchester

Attempts to express the cystic fibrosis transmembrane conductance regulator (CFTR) in Saccharomyces cerevisiae have, until now, yielded relatively low amounts of protein. This protocol and the associated reagents distributed via the Cystic Fibrosis Foundation should allow the preparation of milligram amounts of this 'difficult' eukaryotic membrane protein.

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Cystic Fibrosis Transmembrane Conductance Regulator

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