JoVE Logo
Faculty Resource Center

Sign In

Mapping Inhibitory Neuronal Circuits by Laser Scanning Photostimulation

DOI :

10.3791/3109-v

October 6th, 2011

October 6th, 2011

17,092 Views

1Department of Anatomy and Neurobiology, School of Medicine, University of California, Irvine, 2Department of Biomedical Engineering, School of Engineering, University of California, Irvine

This paper introduces an approach of combining laser scanning photostimulation with whole cell recordings in transgenic mice expressing GFP in limited inhibitory neuron populations. The technique allows for extensive mapping and quantitative analysis of local synaptic circuits of specific inhibitory cortical neurons.

Tags

Inhibitory Neurons

-- Views

Related Videos

article

Laser-scanning Photostimulation of Optogenetically Targeted Forebrain Circuits

article

Inducing Plasticity of Astrocytic Receptors by Manipulation of Neuronal Firing Rates

article

Design, Surface Treatment, Cellular Plating, and Culturing of Modular Neuronal Networks Composed of Functionally Inter-connected Circuits

article

Laser-guided Neuronal Tracing In Brain Explants

article

Rewiring Neuronal Circuits: A New Method for Fast Neurite Extension and Functional Neuronal Connection

article

Chronic Transcranial Electrical Stimulation and Intracortical Recording in Rats

article

Combining Optogenetics with Artificial microRNAs to Characterize the Effects of Gene Knockdown on Presynaptic Function within Intact Neuronal Circuits

article

Optogenetic Entrainment of Hippocampal Theta Oscillations in Behaving Mice

article

Ablation of a Neuronal Population Using a Two-photon Laser and Its Assessment Using Calcium Imaging and Behavioral Recording in Zebrafish Larvae

article

In Vitro Wedge Slice Preparation for Mimicking In Vivo Neuronal Circuit Connectivity

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved