September 2nd, 2013
•The recent development of neuroscience tools that combine genetics and optics, termed "optogenetics", enables control over neural circuit activity with an unprecedented level of spatial and temporal resolution. Here we provide a protocol for integrating in vivo recording with optogenetic manipulation of genetically-defined subsets of prefrontal cortical and subicular pyramidal neurons.
Tags
Related Videos
In vivo Electroporation of Developing Mouse Retina
Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation
Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo
Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI
In vivo Optogenetic Stimulation of the Rodent Central Nervous System
Ex Vivo Optogenetic Dissection of Fear Circuits in Brain Slices
A Simplified Method for Ultra-Low Density, Long-Term Primary Hippocampal Neuron Culture
Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons
Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans
Method for High Speed Stretch Injury of Human Induced Pluripotent Stem Cell-derived Neurons in a 96-well Format
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved