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A Method for High Fidelity Optogenetic Control of Individual Pyramidal Neurons In vivo

DOI :

10.3791/50291-v

13:44 min

September 2nd, 2013

September 2nd, 2013

18,609 Views

1Institute for Behavioral Genetics, University of Colorado Boulder, 2Department of Psychology and Neuroscience, University of Colorado Boulder

The recent development of neuroscience tools that combine genetics and optics, termed "optogenetics", enables control over neural circuit activity with an unprecedented level of spatial and temporal resolution. Here we provide a protocol for integrating in vivo recording with optogenetic manipulation of genetically-defined subsets of prefrontal cortical and subicular pyramidal neurons.

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Optogenetic Control

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