Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry

11.9K Views

13:06 min

November 20th, 2014

DOI :10.3791/51840-v

November 20th, 2014

Transcript

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Keywords In gel Reductive elimination

Chapters in this video

0:05

Title

1:16

Gel Excision and Removal of Gel-derived Contaminants

3:14

In-gel O-glycan Release by Reductive β-Elimination and Desalting on Cation-exchange Chromatography

6:02

Borate Removal, C18 Clean-up and Base Preparation for Permethylation

8:03

Permethylation of Released Glycans C18 Clean-up and Mass Spectrometry Analysis

10:33

Results: Recovery and Mass Spectral Analysis of Permethylated O-linked Glycans

12:30

Conclusion

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