Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry
11.8K Views
•
13:06 min
•
November 20th, 2014
DOI :
November 20th, 2014
•Transcript
Chapters in this video
0:05
Title
1:16
Gel Excision and Removal of Gel-derived Contaminants
3:14
In-gel O-glycan Release by Reductive β-Elimination and Desalting on Cation-exchange Chromatography
6:02
Borate Removal, C18 Clean-up and Base Preparation for Permethylation
8:03
Permethylation of Released Glycans C18 Clean-up and Mass Spectrometry Analysis
10:33
Results: Recovery and Mass Spectral Analysis of Permethylated O-linked Glycans
12:30
Conclusion
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