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Ordering Single Cells and Single Embryos in 3D Confinement: A New Device for High Content Screening

DOI :

10.3791/51880-v

September 18th, 2016

September 18th, 2016

8,396 Views

1Laboratory of Cell Physics, Institut de Science et d'Ingénierie Supramoléculaires (ISIS), CNRS and Université de Strasbourg, 2Development and Stem Cells Program, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS and Université de Strasbourg

We report a device and a new method to study cells and embryos. Single cells are precisely ordered in microcavity arrays. Their 3D confinement is a step towards 3D environments encountered in physiological conditions and allows organelle orientation. By controlling cell shape, this setup minimizes variability reported in standard assays.

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