JoVE Logo
Faculty Resource Center

Sign In

Staining and High-Resolution Imaging of Three-Dimensional Organoid and Spheroid Models

DOI :

10.3791/62280-v

7:35 min

March 27th, 2021

March 27th, 2021

9,553 Views

1Childhood Cancer & Cell Death (C3), Université de Lyon, Université Claude Bernard Lyon 1, INSERM 1052, CNRS 5286, Centre Léon Bérard, Centre de recherche en cancérologie de Lyon (CRCL), Lyon 69373, 2Plateforme Anatomopathologie Recherche, Université de Lyon, Université Claude Bernard Lyon 1, INSERM 1052, CNRS 5286, Centre Léon Bérard, Centre de recherche en cancérologie de Lyon (CRCL), 3Plateforme d’Imagerie cellulaire, Université de Lyon, Université Claude Bernard Lyon 1, INSERM 1052, CNRS 5286, Centre Léon Bérard, Centre de recherche en cancérologie de Lyon (CRCL), 4PerkinElmer S.A.S.

Here, we provide detailed, robust, and complementary protocols to perform staining and subcellular resolution imaging of fixed three-dimensional cell culture models ranging from 100 µm to several millimeters, thus enabling the visualization of their morphology, cell-type composition, and interactions.

Tags

Staining

-- Views

Related Videos

article

Neuronal Cell Cultures from Aplysia for High-Resolution Imaging of Growth Cones

article

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.

article

Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit

article

Isolation of Mammary Epithelial Cells from Three-dimensional Mixed-cell Spheroid Co-culture

article

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins

article

Long-term, High-resolution Confocal Time Lapse Imaging of Arabidopsis Cotyledon Epidermis during Germination

article

Using High Resolution Computed Tomography to Visualize the Three Dimensional Structure and Function of Plant Vasculature

article

Mouse Fetal Whole Intestine Culture System for Ex Vivo Manipulation of Signaling Pathways and Three-dimensional Live Imaging of Villus Development

article

FRET Imaging in Three-dimensional Hydrogels

article

High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved