Sample Preparation for Immunofluorescence Microscopy
4:20
Repositioning of Embryo to an Anatomically Standard Position Using Fiji/ImageJ
7:19
Sample Preparation for Optical Projection Tomography
10:29
Results: Visualization and Analysis of Three and Four-Dimensional Image Data of Mouse Embryos
12:23
Conclusion
Transcript
The organogenesis stages in the mouse embryos remain poorly studied partially due to the difficult visualization of the embryo complex structures. This protocol describes techniques that allow 3D and 4D visualization and analysis of mouse embryos
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Here, we describe computational tools and methods that allow visualization and analysis of three and four-dimensional image data of mouse embryos in the context of axial elongation and segmentation, obtained by in toto optical projection tomography, and by live imaging and whole-mount immunofluorescence staining using multiphoton microscopy.