High Efficiency Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes and Characterization by Flow Cytometry
30.2K Views
•
13:13 min
•
September 23rd, 2014
DOI :
September 23rd, 2014
•Subarna Bhattacharya*1, Paul W. Burridge*2, Erin M. Kropp1, Sandra L. Chuppa1, Wai-Meng Kwok3, Joseph C. Wu2, Kenneth R. Boheler4,5, Rebekah L. Gundry1,6
1Department of Biochemistry, Medical College of Wisconsin, 2Stanford Cardiovascular Institute, Stanford University School of Medicine, 3Department of Anesthesiology, Medical College of Wisconsin, 4Stem Cell and Regenerative Medicine Consortium, LKS Faculty of Medicine, Hong Kong University, 5Division of Cardiology, Johns Hopkins University School of Medicine, 6Cardiovascular Research Center, Biotechnology and Bioengineering Center, Medical College of Wisconsin
Chapters in this video
0:05
Title
1:43
Passaging and Maintenance of Undifferentiated hPSCs in Monolayer Culture
5:18
Cardiomyocyte Induction of hPSCs by Selective Modulation of the Wnt Pathway
8:22
Fixation and Permeabilization of Cells for Intracellular Antigen Staining
9:25
Preparation of Cells for Flow Cytometry and Flow Cytometric Analysis
10:34
Characterization of Cardiomyocytes by Flow Cytometry and Electrophysiology
12:26
Conclusion
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