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Derivation of Cardiac Progenitor Cells from Embryonic Stem Cells

DOI :

10.3791/52047-v

January 12th, 2015

January 12th, 2015

7,932 Views

1Cardiac Surgery, University of Michigan, 2Leon H Charney Division of Cardiology, New York University School of Medicine

In this protocol, derivation of cardiac progenitor cells from both mouse and human embryonic stem cells will be illustrated. A major strategy in this protocol is to enrich cardiac progenitor cells with flow cytometry using fluorescent reporters engineered into the embryonic stem cell lines.

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Cardiac Progenitor Cells

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