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Reporter-based Growth Assay for Systematic Analysis of Protein Degradation

DOI :

10.3791/52021-v

November 6th, 2014

November 6th, 2014

10,580 Views

1Department of Biological Chemistry, The Hebrew University of Jerusalem

Here we describe a robust biological assay for quantifying the relative rate of proteolysis by the ubiquitin-proteasome system. The assay readout is yeast growth rate in liquid culture, which is dependent on the cellular levels of a reporter protein comprising a degradation signal fused to an essential metabolic marker.

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Reporter based Growth Assay

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