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Assays for the Degradation of Misfolded Proteins in Cells

DOI :

10.3791/54266-v

August 28th, 2016

August 28th, 2016

11,476 Views

1Department of Cancer Biology, University of Pennsylvania Perelman School of Medicine, 2Department of Systems Pharmacology and Translational Therapeutics, University of Pennsylvania Perelman School of Medicine

This report describes protocols for measuring degradation rates of misfolded proteins by either western blot or fluorescence-based assays. The methods can be applied to analysis of other misfolded proteins and for high throughput screening.

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