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Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae

DOI :

10.3791/53975-v

April 18th, 2016

April 18th, 2016

27,936 Views

1Department of Biology, Ball State University, 2Bioproduct Research & Development, Eli Lilly and Company

Protein abundance reflects the rates of both protein synthesis and protein degradation. This article describes the use of cycloheximide chase followed by western blotting to analyze protein degradation in the model unicellular eukaryote, Saccharomyces cerevisiae (budding yeast).

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Cycloheximide Chase Analysis

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