JoVE Logo
Faculty Resource Center

Sign In

Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae

DOI :

10.3791/53975-v

April 18th, 2016

April 18th, 2016

27,342 Views

1Department of Biology, Ball State University, 2Bioproduct Research & Development, Eli Lilly and Company

Protein abundance reflects the rates of both protein synthesis and protein degradation. This article describes the use of cycloheximide chase followed by western blotting to analyze protein degradation in the model unicellular eukaryote, Saccharomyces cerevisiae (budding yeast).

-- Views

Related Videos

article

Evaluation of Lipid Droplet Size and Fusion in Bovine Hepatic Cells

article

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro

article

Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats

article

Direct Synthesis of EM-Visible Gold Nanoparticles in Cells for Protein Localization Analysis with Well-Preserved Ultrastructure

article

A Precise and Quantifiable Method for Collecting Hemolymph from Small Arthropods

article

Two-Step Tag-Free Isolation of Mitochondria for Improved Protein Discovery and Quantification

article

Author Spotlight: Exploring Cellular Zinc Regulation Through ZnT1 Functionality

article

Author Spotlight: Advancing Mitochondrial Research – mtHyper7 Biosensor for Subcellular Analysis

article

Decellularization-Based Quantification of Skeletal Muscle Fatty Infiltration

article

Imaging ATG9A, a Multi-Spanning Membrane Protein

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved