October 31st, 2014
•We here describe a fluorescence based primer extension method to determine transcriptional starting points from bacterial transcripts and RNA processing in vivo using an automated gel sequencer.
Related Videos
Proboscis Extension Response (PER) Assay in Drosophila
Mesoscopic Fluorescence Tomography for In-vivo Imaging of Developing Drosophila
Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources
Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA
Single-molecule Imaging of Gene Regulation In vivo Using Cotranslational Activation by Cleavage (CoTrAC)
A Quantitative Assay to Study Protein:DNA Interactions, Discover Transcriptional Regulators of Gene Expression, and Identify Novel Anti-tumor Agents
A Proteoliposome-Based Efflux Assay to Determine Single-molecule Properties of Cl- Channels and Transporters
Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability
Profiling of H3K4me3 Modification in Plants using Cleavage under Targets and Tagmentation
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved