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Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR

16.5K Views

14:14 min

December 6th, 2014

DOI :

10.3791/52184-v

December 6th, 2014


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Keywords T cell Receptor Excision Circles TRECs

Chapters in this video

0:05

Title

2:34

Preparation of Triple-insert Plasmid

4:49

Standard Curve Preparation and Real Time PCR for Quantification of TRECS and KRECS

7:25

Calculations

11:59

Results: TREC and KREC Quantification in Healthy Individuals

12:54

Conclusion

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