Cell Culture, Treatments, and Coverslip Preparation
1:18
Pre-Extraction and Fixation
2:13
Permeabilization and Immunostaining
3:53
Image Analysis
4:16
Results: Visualization of DNA Double-Strand End Resection During S to G2 Phase of the Cell Cycle Using an Immunofluorescence-Based Method
6:05
Conclusion
Transcript
This protocol allows for in cellulo quantification of DNA and resection following DNA damage. This technique's main advantage is the cell cycle marking which restricts the cells to S and G2 phase, ensuring the bromodeoxyuridine signal results from
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In the present protocol, we demonstrate how to visualize DNA double-strand end resection during S/G2 phase of the cell cycle using an immunofluorescence-based method.