JoVE Logo
Faculty Resource Center

Sign In

Determination of Immune Cell Identity and Purity Using Epigenetic-Based Quantitative PCR

DOI :

10.3791/60465-v

8:02 min

February 19th, 2020

February 19th, 2020

6,626 Views

1Cell Biology, Life Sciences Solutions, Thermo Fisher Scientific, 2California Polytechnic State University (Cal Poly), 3Epiontis GmbH, Precision for Medicine

Here we describe a robust method of determining immune cell identity and purity through epigenetic signatures detected using quantitative PCR (qPCR). DNA demethylation at a specific locus serves as a unique identifier for a particular cell type and allows for identification of CD8+, regulatory, or Th17 T cells.

Tags

Immune Cell Identity

-- Views

Related Videos

article

Quantitative Assessment of Immune Cells in the Injured Spinal Cord Tissue by Flow Cytometry: a Novel Use for a Cell Purification Method

article

Isolation of Functional Cardiac Immune Cells

article

Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy

article

Quantitative Measurement of the Immune Response and Sleep in Drosophila

article

Generation of Lymph Node-fat Pad Chimeras for the Study of Lymph Node Stromal Cell Origin

article

Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids

article

Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry

article

Quantitative PCR of T7 Bacteriophage from Biopanning

article

Determination of Regulatory T Cell Subsets in Murine Thymus, Pancreatic Draining Lymph Node and Spleen Using Flow Cytometry

article

Assessing the Cellular Immune Response of the Fruit Fly, Drosophila melanogaster, Using an In Vivo Phagocytosis Assay

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved