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Determination of Immune Cell Identity and Purity Using Epigenetic-Based Quantitative PCR (Video) | JoVE

DOI :

10.3791/60465-v

8:02 min

February 19th, 2020

February 19th, 2020

6,578 Views

1Cell Biology, Life Sciences Solutions, Thermo Fisher Scientific, 2California Polytechnic State University (Cal Poly), 3Epiontis GmbH, Precision for Medicine

Here we describe a robust method of determining immune cell identity and purity through epigenetic signatures detected using quantitative PCR (qPCR). DNA demethylation at a specific locus serves as a unique identifier for a particular cell type and allows for identification of CD8+, regulatory, or Th17 T cells.

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Immune Cell Identity

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