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JoVE Journal

Bioengineering

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Nutrient Regulation by Continuous Feeding for Large-scale Expansion of Mammalian Cells in Spheroids

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11:01 min

September 25th, 2016

September 25th, 2016

7,652 Views

0:05

Title

1:05

Assemble the Continuous Feeding System

4:30

Spheroid Formation

6:04

Continuous Feeding Culture and Adjusted Feed Rate

8:50

Results: Improved Growth Curve and Cell Yields from Cells Grown in Stirred Suspension Bioreactors

10:05

Conclusion

Transcript

The overall goal of this process is to culture large number of cells under controlled nutrient conditions. Using conventional equipment found in a cell culture laboratory. The associated algorithms allow the control and manipulation of nutrients w

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Nutrient regulation using continuous growth adjusted feeding improves growth rates of mammalian cell spheroids compared to intermittent batch feeding for cultures in stirred suspension bioreactors. This study demonstrates the methods required for establishing simple adjusted rate fed cultures.

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