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Generation of Multicue Cellular Microenvironments by UV-Photopatterning of Three-Dimensional Cell Culture Substrates

DOI :

10.3791/63988-v

9:30 min

June 2nd, 2022

June 2nd, 2022

1,966 Views

1Department of Biomedical Engineering, Eindhoven University of Technology, 2Institute for Complex Molecular Systems, Eindhoven University of Technology

Traditionally, cell culture is performed on planar substrates that poorly mimic the natural environment of cells in vivo. Here we describe a method to produce cell culture substrates with physiologically relevant curved geometries and micropatterned extracellular proteins, allowing systematic investigations into cellular sensing of these extracellular cues.

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UV photopatterning

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