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Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification

DOI :

10.3791/52423-v

February 19th, 2015

February 19th, 2015

10,791 Views

1Centre for Vascular Research, University of New South Wales, 2School of Medical Sciences, University of New South Wales

Here, we present a protocol to continuously quantify cell adhesion and de-adhesion processes with high temporal resolution in a non-invasive manner by cell-substrate impedance and live cell imaging analyses. These approaches reveal the dynamics of cell adhesion/de-adhesion processes triggered by matrix modification and their temporal relationship to adhesion-dependent signaling events.

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Keywords Cell matrix Adhesion

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