February 16th, 2015
•This article describes a yeast growth-based assay for the determination of genetic requirements for protein degradation. It also demonstrates a method for rapid extraction of yeast proteins, suitable for western blotting to biochemically confirm degradation requirements. These techniques can be adapted to monitor degradation of a variety of proteins.
Related Videos
Dissection of Saccharomyces Cerevisiae Asci
Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae
Microarray Analysis for Saccharomyces cerevisiae
A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast Saccharomyces cerevisiae
Expression and Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein in Saccharomyces cerevisiae
Biochemical and High Throughput Microscopic Assessment of Fat Mass in Caenorhabditis Elegans
Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae
Coupled Assays for Monitoring Protein Refolding in Saccharomyces cerevisiae
Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein Expressed in Saccharomyces cerevisiae
Rapid Identification of Chemical Genetic Interactions in Saccharomyces cerevisiae
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved