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Super-resolution Imaging of the Natural Killer Cell Immunological Synapse on a Glass-supported Planar Lipid Bilayer

DOI :

10.3791/52502-v

9:56 min

February 11th, 2015

February 11th, 2015

11,958 Views

1Center for Human Immunobiology, Texas Children's Hospital, 2Department of Pediatrics, Baylor College of Medicine, 3Department of Pathology and Immunology, Baylor College of Medicine

We describe here a combination of the glass-supported lipid bilayer technique of forming immunological synapses with the super-resolution imaging technique of stimulated emission depletion (STED) microscopy. The goal of this protocol is to provide users with the instructions necessary to successfully carry out these two techniques.

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Super resolution Imaging

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