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Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy

DOI :

10.3791/51100-v

10:00 min

March 24th, 2014

March 24th, 2014

77,686 Views

1Center for Human Immunobiology, Texas Children's Hospital and Baylor College of Medicine

Here we illustrate the protocol for imaging by two-color STED nanoscopy the cytotoxic immune synapse of NK cells recapitulated on glass. Using this method we obtain sub-100 nm resolution of synapse proteins and the cytoskeleton.

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Immunological Synapse

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