Human Dupuytren's Ex Vivo Culture for the Study of Myofibroblasts and Extracellular Matrix Interactions
10.4K Views
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08:59 min
•
April 18th, 2015
DOI :
April 18th, 2015
•Sofia Karkampouna1, Peter Kloen2, Miryam C. Obdeijn3, Scott M. Riester4, Andre J. van Wijnen4,5, Marianna Kruithof-de Julio1,6
1Department of Molecular Cell Biology, Cancer Genomics Centre and Centre for Biomedical Genetics, Leiden University Medical Center, 2Department of Orthopedic Surgery, Academic Medical Center, 3Department of Plastic, Reconstructive and Hand Surgery, Academic Medical Center, 4Department of Orthopedic Surgery, Mayo Clinic, 5Department of Biochemistry and Molecular Biology, Mayo Clinic, 6Department of Dermatology, Leiden University Medical Center
Chapters in this video
0:05
Title
2:06
Tissue Preparation and Ex Vivo Tissue Culture Setup
5:27
Combined Second Harmonic Generation (Collagen) and Two-photon Excited Fluorescence (Elastin) Imaging on Ex Vivo Tissue during Culture
6:39
Results: Effects on 3D Extracellular Matrix Composition upon Tissue Manipulation by Compound Treatments and Viral-mediated Gene Modulation
8:17
Conclusion
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