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High-Throughput Contractile Measurements of Hydrogel-Embedded Intact Mouse Muscle Fibers Using an Optics-Based System

DOI :

10.3791/65103-v

7:35 min

May 5th, 2023

May 5th, 2023

702 Views

1Department of Physiology, Amsterdam UMC, 2Amsterdam Cardiovascular Sciences, Heart Failures and Arrhythmias, Amsterdam UMC, 3Amsterdam Movement Sciences, Tissue Function and Regeneration, Amsterdam UMC, 4Discipline of Child and Adolescent Health, Faculty of Health and Medicine, University of Sydney

Skeletal muscle function can be assessed by quantifying the contractility of isolated muscle fibers, traditionally using laborious, low-throughput approaches. Here, we describe an optics-based, high-throughput method to quantify the contractility of hydrogel-embedded muscle fibers. This approach has applications for drug screening and therapeutic development.

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High throughput

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