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Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

DOI :

10.3791/52739-v

April 16th, 2015

April 16th, 2015

49,643 Views

1Department of Microbiology and Immunology, The Chicago Medical School, Rosalind Franklin University of Medicine and Science

Naïve CD4+ T cells polarize to various subsets depending on the environment at the time of activation. The differentiation of naïve CD4+ T cells to various effector subsets can be achieved in vitro through the addition of T cell receptor stimuli and specific cytokine signals.

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