JoVE Logo
Faculty Resource Center

Sign In

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets Video (Video) | JoVE

DOI :

10.3791/52739-v

April 16th, 2015

April 16th, 2015

49,887 Views

1Department of Microbiology and Immunology, The Chicago Medical School, Rosalind Franklin University of Medicine and Science

Naïve CD4+ T cells polarize to various subsets depending on the environment at the time of activation. The differentiation of naïve CD4+ T cells to various effector subsets can be achieved in vitro through the addition of T cell receptor stimuli and specific cytokine signals.

-- Views

Related Videos

article

Retroviral Transduction of T-cell Receptors in Mouse T-cells Video (Video) | JoVE

article

PiggyBac Transposon System Modification of Primary Human T Cells Video (Video) | JoVE

article

Isolation of Precursor B-cell Subsets from Umbilical Cord Blood Video (Video) | JoVE

article

Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation Video (Video) | JoVE

article

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes Video (Video) | JoVE

article

In Vitro Assay to Evaluate the Impact of Immunoregulatory Pathways on HIV-specific CD4 T Cell Effector Function Video (Video) | JoVE

article

Transplantation of Tail Skin to Study Allogeneic CD4 T Cell Responses in Mice Video (Video) | JoVE

article

Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice Video (Video) | JoVE

article

Kupffer Cell Isolation for Nanoparticle Toxicity Testing Video (Video) | JoVE

article

Isolation and Ex Vivo Culture of Vδ1+CD4+γδ T Cells, an Extrathymic αβT-cell Progenitor Video (Video) | JoVE

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved