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Imaging CD4 T Cell Interstitial Migration in the Inflamed Dermis

DOI :

10.3791/53585-v

March 25th, 2016

March 25th, 2016

10,394 Views

1David H. Smith Center for Vaccine Biology and Immunology, Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, NY

The mechanisms that govern the interstitial motility of CD4 effector T cells at sites of inflammation are relatively unknown. We present a non-invasive approach to visualize and manipulate in vitro-primed CD4 T cells in the inflamed ear dermis, allowing for study of the dynamic behavior of these cells in situ.

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