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Design and Construction of Artificial Extracellular Matrix AECM Proteins from Escherichia coli for Skin Tissue Engineering Video (Video) | JoVE

DOI :

10.3791/52845-v

June 11th, 2015

June 11th, 2015

8,664 Views

1School of Materials Science and Engineering, Nanyang Technological University

Recombinant technologies have enabled material designers to create novel artificial proteins with customized functionalities for tissue engineering applications. For example, artificial extracellular matrix proteins can be designed to incorporate structural and biological domains derived from native ECMs. Here, we describe the construction and purification of aECM proteins containing elastin-like repeats.

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