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Engineering Three-dimensional Epithelial Tissues Embedded within Extracellular Matrix

DOI :

10.3791/54283-v

July 10th, 2016

July 10th, 2016

7,314 Views

1Chemical and Biological Engineering, Princeton University, 2Molecular Biology, Princeton University

This manuscript describes a soft lithography-based technique to engineer uniform arrays of three-dimensional (3D) epithelial tissues of defined geometry surrounded by extracellular matrix. This method is amenable to a wide variety of cell types and experimental contexts and allows for high-throughput screening of identical replicates.

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