Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications
9.0K Views
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08:41 min
•
December 4th, 2017
DOI :
December 4th, 2017
•Matthias Becker1,2, Janita A. Maring1,2, Barbara Oberwallner2, Benjamin Kappler2, Oliver Klein1,2, Volkmar Falk2,3,4,5, Christof Stamm2,3,4
1Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 2Berlin-Brandenburg Center for Regenerative Therapies (BCRT), 3German Center for Cardiovascular Research (DZHK), 4Deutsches Herzzentrum Berlin (DHZB), 5Department of Cardiovascular Surgery, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health
Chapters in this video
0:05
Title
0:43
Sectioning Human Myocardium into Slices
2:27
Decellularization of Human Myocardium Slices
4:20
Processing the Decellularized cECM to a Powder
5:50
Enzyme-based Homogenization of cECM Micro-particles
6:56
Results: Effective Removal of Cellular Material Enhances Analysis
8:10
Conclusion
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