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Production of Extracellular Matrix Fibers via Sacrificial Hollow Fiber Membrane Cell Culture

DOI :

10.3791/58791-v

February 2nd, 2019

February 2nd, 2019

7,178 Views

1Cell & Molecular Biology Program, University of Arkansas, 2Department of Biomedical Engineering, University of Arkansas, 3Ralph E. Martin Department of Biomedical Engineering, University of Arkansas

The goal of this protocol is production of whole extracellular matrix fibers targeted for wound repair which are suitable for preclinical evaluation as part of a regenerative scaffold implant. These fibers are produced by the culture of fibroblasts on hollow fiber membranes and extracted by dissolution of the membranes.

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Extracellular Matrix Fibers

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