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Silencing of BRCA2 to Identify Novel BRCA2-regulated Biological Functions in Cultured Human Cells

DOI :

10.3791/52849-v

August 12th, 2015

August 12th, 2015

8,871 Views

1Institute of Biomembranes and Bioenergetics, National Research Council

Gene silencing by siRNA represents a convenient experimental strategy to analyze BRCA2-dependent biological functions with immediate implications to better understand cancer biology. A method to efficiently silence BRCA2, along with the experimental procedure to detect and quantify changes in BRCA2 protein expression by immunoblotting in human cell lines, is presented.

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BRCA2

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