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JoVE Journal

Immunology and Infection

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An In Vitro Model for Measuring Immune Responses to Malaria in the Context of HIV Co-infection

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08:14 min

October 6th, 2015

October 6th, 2015

10,217 Views

0:05

Title

0:57

Parasite Synchronization and Preparation for Co-culture

2:27

Human Peripheral Blood Mononuclear Cell (PBMC) Isolation

3:42

Malaria/HIV Co-infection Culture and Detection of Malaria-specific Immune Responses

4:52

Cell-specific Cytokine Production by PBMC Co-culture with P. falciparum Infected RBC

6:57

Results: Representative IFNγ Production by Natural Killer T Cells

7:38

Conclusion

Human co-infection is difficult to replicate in vitro. However, human malaria parasites can readily be cultured in vitro, as can freshly isolated human peripheral blood mononuclear cells naturally infected with HIV. This provides an excellent model for studying early immune responses to malaria parasites in the context of HIV co-infection.

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