JoVE Logo

Sign In

Measuring Naturally Acquired Phagocytosis-Inducing Antibodies to Plasmodium falciparum Parasites by a Flow Cytometry-Based Assay

6.3K Views

09:57 min

August 6th, 2020

DOI :

10.3791/61538-v

August 6th, 2020


Explore More Videos

Phagocytosis

Chapters in this video

0:04

Introduction

0:33

THP-1 and Infected Erythrocyte (IE) Culture Setup

1:58

Parasite Staining and Opsonization Plate Preparation

3:47

THP-1 Cell and Phagocytosis Plate Preparation

4:35

Phagocytosis Assay

6:21

Flow Cytometry Acquisition and Analysis

7:34

Results: Representative Evaluation of Parasite-IE Opsonization and Phagocytosis

9:21

Conclusion

Related Videos

article

10:38

High Yield Purification of Plasmodium falciparum Merozoites For Use in Opsonizing Antibody Assays

22.9K Views

article

07:16

In Vivo Assessment of Rodent Plasmodium Parasitemia and Merozoite Invasion by Flow Cytometry

9.9K Views

article

08:14

An In Vitro Model for Measuring Immune Responses to Malaria in the Context of HIV Co-infection

10.2K Views

article

13:06

Analysis of Single-cell Gene Transcription by RNA Fluorescent In Situ Hybridization (FISH)

15.4K Views

article

09:43

Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry

6.7K Views

article

05:28

Standard Membrane Feeding Assay for the Detection of Plasmodium falciparum Infection in Anopheles Mosquito Vectors

2.7K Views

article

08:20

In Vitro Assay of Plasmodium-Infected Red Blood Cell Killing by Cytotoxic Lymphocytes

2.0K Views

article

07:04

Detection of Plasmodium Sporozoites in Anopheles Mosquitoes using an Enzyme-linked Immunosorbent Assay

1.9K Views

article

01:34

Quantitative Flow Cytometric Measurement of Fungal Spore Phagocytosis by Human Phagocytes

69 Views

article

01:47

An Assay for Quantifying Antibody-Mediated Phagocytosis of Parasite-Infected Erythrocytes

107 Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved