Measuring Naturally Acquired Phagocytosis-Inducing Antibodies to Plasmodium falciparum Parasites by a Flow Cytometry-Based Assay
6.3K Views
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09:57 min
•
August 6th, 2020
DOI :
August 6th, 2020
•1Centre for Medical Parasitology, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, 2West African Centre for Cell Biology of Infectious Pathogens, Department of Biochemistry, Cell and Molecular Biology, University of Ghana, 3Department of Immunology, Noguchi Memorial Institute for Medical Research, 4Centre for Medical Parasitology, Department of Infectious Diseases, Rigshospitalet
Chapters in this video
0:04
Introduction
0:33
THP-1 and Infected Erythrocyte (IE) Culture Setup
1:58
Parasite Staining and Opsonization Plate Preparation
3:47
THP-1 Cell and Phagocytosis Plate Preparation
4:35
Phagocytosis Assay
6:21
Flow Cytometry Acquisition and Analysis
7:34
Results: Representative Evaluation of Parasite-IE Opsonization and Phagocytosis
9:21
Conclusion
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