JoVE Logo
Faculty Resource Center

Sign In

Studying Protein Function and the Role of Altered Protein Expression by Antibody Interference and Three-dimensional Reconstructions

DOI :

10.3791/53049-v

April 21st, 2016

April 21st, 2016

6,555 Views

1Institute for Biochemistry, Emil-Fischer Centre, University of Erlangen-Nuremberg, 2Department of Biology, Animal Physiology, University of Erlangen-Nuremberg

Controlling protein expression is not only essential to every organism alive, but also an important strategy to investigate protein functions in cellular models. The protocol presented shows the application of antibody interference in mammalian cells including primary hippocampal neurons and demonstrates the use of three-dimensional reconstructions in studying protein function.

Tags

Keywords Protein Function

-- Views

Related Videos

article

Thermodynamics of Membrane Protein Folding Measured by Fluorescence Spectroscopy

article

Alginate Hydrogels for Three-Dimensional Organ Culture of Ovaries and Oviducts

article

The Three-Dimensional Human Skin Reconstruct Model: a Tool to Study Normal Skin and Melanoma Progression

article

Planar and Three-Dimensional Printing of Conductive Inks

article

High-throughput Protein Expression Generator Using a Microfluidic Platform

article

Microfluidic Mixers for Studying Protein Folding

article

Investigating the Three-dimensional Flow Separation Induced by a Model Vocal Fold Polyp

article

Characterization of Complex Systems Using the Design of Experiments Approach: Transient Protein Expression in Tobacco as a Case Study

article

Human Cartilage Tissue Fabrication Using Three-dimensional Inkjet Printing Technology

article

Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved