Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve

12.8K Views

•

09:13 min

•

June 14th, 2017

DOI :

10.3791/55762-v

June 14th, 2017

•

Cristina Banfi¹, Anna Guarino², Maura Brioschi¹, Stefania Ghilardi¹, Valeria Mastrullo¹, Elena Tremoli¹, Gianluca Polvani²^,³^,⁴

¹Centro Cardiologico Monzino IRCCS, ²Cardiovascular Tissue Bank of Milan, Centro Cardiologico Monzino IRCCS, ³Department of Clinical Sciences and Community Health, Cardiovascular Section, University of Milan, ⁴Department of Cardiovascular Disease, Development and Innovation Cardiac Surgery Unit, Centro Cardiologico Monzino IRCCS

Summary

Explore More Videos

Keywords Protein Extraction

Mitral Valve

Cardiac Valve Disease

Anterior Mitral Valve Leaflet

Posterior Mitral Valve Leaflet

Commissures

Dissection

Protein Extraction Workflow

Chapters in this video

0:05

Title

1:17

Mitral Valve Preparation

3:39

Protein Extraction

7:20

Results: Four Newly Identified Mitral Valve Proteins

8:28

Conclusion

Related Videos

09:58

An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides

15.5K Views

12:03

Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells

9.4K Views

11:17

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

34.7K Views

07:57

Sampling and Pretreatment of Tooth Enamel Carbonate for Stable Carbon and Oxygen Isotope Analysis

13.8K Views

11:13

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy

11.0K Views

10:00

Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering (SEC-MALS)

53.5K Views

05:22

Transverse Sectioning of Mature Rice (Oryza sativa L.) Kernels for Scanning Electron Microscopy Imaging Using Pipette Tips as Immobilization Support

3.5K Views

07:27

Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry

5.0K Views

07:24

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins

1.7K Views

05:46

Ultrasonic-Assisted Extraction of Cannabidiolic Acid from Cannabis Biomass

5.9K Views