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Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions

DOI :

10.3791/53115-v

October 13th, 2015

October 13th, 2015

8,359 Views

1Institute for Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf, 2UKE Microscopy Imaging Facility, University Medical Center Hamburg-Eppendorf

Effector translocation into host cells via a type III secretion system is a common virulence strategy among gram-negative bacteria. A beta-lactamase effector fusion based assay for quantitative analysis of translocation was applied. In Yersinia infected cells, conversion of a FRET reporter by the beta-lactamase is monitored using laser scanning microscopy.

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