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Applying Fluorescence Resonance Energy Transfer (FRET) to Examine Effector Translocation Efficiency by Coxiella burnetii during siRNA Silencing

DOI :

10.3791/54210-v

July 6th, 2016

July 6th, 2016

10,474 Views

1Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne

Investigating the interactions between bacterial pathogens and their hosts is an important area of biological research. Here, we describe the necessary techniques to measure effector translocation by Coxiella burnetii during siRNA gene silencing using BlaM substrate.

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